Data achieved by the standard fingerprinting methods, terminal restriction fragment length polymorphism (T-RFLP) and capillary electrophoresis single strand conformation polymorphism (CE-SSCP) on the same samples followed the same trend as qPCR data. The microbial community analysis via qPCR allowed a precise monitoring of the evolution of total biomass as well as abundance of specific species. Stirred tank bioreactors for the leaching of copper concentrate, housing a consortium of acidophilic, moderately thermophilic bacteria, relevant in several bioleaching operations, served as a model system. Therefore, our study focused on the development of novel quantitative real-time PCR (qPCR) assays for the quantification of Acidithiobacillus caldus, Leptospirillum ferriphilum, Sulfobacillus thermosulfidooxidans, and Sulfobacillus benefaciens and comparison of the results with data from other common molecular monitoring methods in order to evaluate their accuracy and specificity. Suitable methods are, however, limited as they are usually not adapted to bioleaching samples and often no taxon-specific assays are available in the literature for these types of consortia. Monitoring of the microbial community in bioleaching processes is essential in order to control process parameters and enhance the leaching efficiency. 2Bureau de Recherches Géologiques et Minières, Orléans, France.1Resource Geochemistry, Federal Institute for Geosciences and Natural Resources, Hannover, Germany.Sabrina Hedrich 1*, Anne-Gwenaëlle Guézennec 2, Mickaël Charron 2, Axel Schippers 1 and Catherine Joulian 2
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